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Structural characteristics of colloidal gold and commonly used detection techniques

(1) The structure of colloidal ore gold
Colloidal Gold, also known by gold sol, is a suspended of pure gold particles. This is formed when the gold salt has been reduced back to original gold. The essential gold core, also known as the atomic gold Au, is found within colloidal particles of colloidal. It’s surrounded by a double layer of ions. For the maintenance of the suspension of colloidal silver between sols and days, the layer with negative ions (AuC122-) is connected to its surface. Colloid gold particles’ primary gold core isn’t ideal for spherical symmetry. Although the colloidal gold particles smaller than 30nm are usually spherical, larger colloidal gem particles that are greater in number are often elliptical. A electron microscope allows you to observe colloidal-gold’s particle morphology.
(2) Some characteristics of colloidal Gold
1. Colloidal nature. Most colloidal gold particles measure between 100 and 1000 nm in size. These tiny gold particles can be suspended in the liquid in an even distribution to form a colloidal solution. Colloid gold therefore has different properties, particularly in terms of sensitivity to electrodelytes. In order to break the solid state, the electrolyte could destroy the permanent hydrohydration layer on the colloidal golden particles. Some macromolecular substances, including proteins, can protect and improve the stability of colloidal golden.
It is an often used labeling technology. It’s a brand new immunolabeling technique that utilizes colloidal gold for tracer markers of antigens or antibodies. It offers many advantages. It’s been extensively used in biological studies for many years. The label is used by almost every immunoblotting technique. This label is also useful in electron microscopy and flow cytometry as well.
Chloroauric acid (HAuCl4) is used to make colloidal gold. When reducing agents are applied, like sodium citrates, white phosphorus or ascorbic acids, tannic, tannic, etc., the resulting gold particles can be made into a specified size. Then, static electricity creates a stable state of colloidal. The stable state of colloidal colloidal is formed by a negatively-charged hydrophobic glue. It can be positively charged when it is placed in an alkaline environment. This allows colloidal gold to form strong bonds with negatively charged molecules of protein molecules. Since this bond is electrostatic it doesn’t affect the biological attributes of the protein.
A coating procedure that allows proteins to be adsorbed on the colloidal-gold particles’ surface is called Colloidal Gold Labeling. Adsorption can be explained by the existence of a negative charge on the surface colloidal-gold particles. The negatively charged group of the protein forms strong bonds with them due to electrostatic attraction. Chloroauric Acid can be used to make colloidal silver particles, which are of different sizes. This cylindrical particle can be used to absorb protein. It is non-covalently compatible with staphylococcal Protein A, immunoglobulins. Because of this, it is a great tool in both basic research and clinical experimentation.
Common detecting technology
Immunocolloid Gold Staining
Staining cell sections and suspensions of tissue can be done with colloidal -labeled antibodies. Also, the silver developer is made from colloidal -labeled silver particles.
Immunocolloid gold electron microscope staining
The antibodies and anti-antibodies labels with colloidal silver can be combined with negatively stained virus samples, or ultrathin tissues sections to make them negatively stained. It can be used in the detection and observation of viruses morphology.
Dot immunogold filtration
A micropore membrane, such as a membrane, can be used as a carrier. Once the antigen has been identified, the material to be tested should then have its sample sealed.
Colloidal gold immunochromatography
The membrane is coated with the antibody specific to it in a stripe form. On the binding pads, the colloidal golden labeling reagent or monoclonal antibody is adsorbed. To test the specimen, add it to the bottom of the test strip. With the naked eye, you can observe the color development results on the test-strip. The diagnostic test strip is extremely convenient because it uses the same method.
The rapid development of standard gold standard technology
Once the antibody has been attached to one particular region of the acid cell membrane, it is then fixed. If one side of the acid cellulose is placed in the sample (urine/serum), it will be moved along the membrane via capillary motion Move. As the sample moves to the location where the antibody has been fixed, the antigen will attach to the antibody. The inclusion of gold particles in the sample has high electron density, which is a characteristic of gold-labeled antibodies. This was visible by the naked eye when red spots appeared as a result of the accumulation of these markers at the ligands. This principle underlies the rapid-gold label detection technique. If the antigens used (monoclonal antibodies or polyclonal proteins), have the required sensitivity and specificity to be produced quickly and accurately, then the production can proceed according to the highest standard. This is what defines the highest quality standard in standard reagents.

Lemondedudroit, Lemondedudroit advanced Material Tech Co., Ltd., is a Colloidalgold producer who has over twelve years experience in chemical product development and research. Please contact us to inquire about high-quality Colloidal golden.

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